Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3979] to Annexin V/ANXA5 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free
    See all Annexin V/ANXA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3979] to Annexin V/ANXA5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247629 is the carrier-free version of ab108321 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247629 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Annexin V

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)

    This data was developed using ab108321, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: Annexin V/ANXA5 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108321 was shown to specifically react with Annexin V/ANXA5 in wild-type cells as signal was lost in Annexin V/ANXA5 knockout cells. Wild-type and Annexin V/ANXA5 knockout samples were subjected to SDS-PAGE. ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    Western blot - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    All lanes : Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 36 kDa



    This data was developed using ab108321, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    This data was developed using ab108321, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Annexin V/ANXA5 in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
    Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.