Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free (ab247629)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3979] to Annexin V/ANXA5 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Annexin V/ANXA5 antibody [EPR3979] - BSA and Azide free
See all Annexin V/ANXA5 primary antibodies -
Description
Rabbit monoclonal [EPR3979] to Annexin V/ANXA5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247629 is the carrier-free version of ab108321 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247629 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Annexin V
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3979 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab108321, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin V/ANXA5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab108321 observed at 35 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108321 was shown to specifically react with Annexin V/ANXA5 in wild-type cells as signal was lost in Annexin V/ANXA5 knockout cells. Wild-type and Annexin V/ANXA5 knockout samples were subjected to SDS-PAGE. ab108321 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDaThis data was developed using ab108321, the same antibody clone in a different buffer formulation.
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This data was developed using ab108321, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Annexin V/ANXA5 in paraffin embedded Human gastric tissue, using ab108321 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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