Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24127-89] to IFNGR1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, ICC
  • Reacts with: Mouse

Overview

  • Product name

    Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free
    See all IFNGR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24127-89] to IFNGR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, ICCmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: EL4 whole cell lysate. ICC: EL4 cells Flow Cyt: EL4 cells; Mouse blood cells. IP: EL4 cells.

  • General notes

    ab280358 is the carrier-free version of ab280353. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280358 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    Western blot - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    All lanes : Anti-IFNGR1 antibody [EPR24127-89] (ab280353) at 1/1000 dilution

    Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 54 kDa
    Observed band size: 70-95 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280353, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: 4T1(PMID:12154047)

    Exposure time: 74 seconds

     

  • Immunocytochemistry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    Immunocytochemistry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)

    This data was developed using ab280353, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 cells labelling IFNGR1 with ab280353 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cell line. Low expression control:4T1 (PMID: 12154047) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)

    This data was developed using ab280353, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4T1 (mouse mammary gland carcinoma epithelial cell, left)/ EL4 (mouse lymphoma T lymphocyte, right) cells labelling IFNGR1 with ab280353 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Low expression: 4T1(PMID:12154047) Gated on viable cells.

  • Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    Flow Cytometry - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)

    This data was developed using ab280353, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse blood cells cells labelling IFNGR1 with ab280353 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)
    Immunoprecipitation - Anti-IFNGR1 antibody [EPR24127-89] - BSA and Azide free (ab280358)

    This data was developed using ab280353, the same antibody clone in a different buffer formulation.

    IFNGR1 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug with ab280353 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280353 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug

    Lane 2: ab280353 IP in EL4 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280353 in EL4 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

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