Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)
![Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)](https://www.abcam.com/ps/products/223/ab223545/Images/ab223545-282799-anti-tlr2-antibody-epr20303-immunoprecipitation.jpg "Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20303] to TLR2 - Low endotoxin, Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free
See all TLR2 primary antibodies -
Description
Rabbit monoclonal [EPR20303] to TLR2 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat spleen lysates; RAW 264.7 whole cell lysates untreated and treated with 1 µg/ml lipopolysaccharides for 6 hours . IP: RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate.
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General notes
ab223545 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20303 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)
This IP data was generated using the same anti-TLR2 antibody clone [EPR20303] in a different buffer formulation ( cat# ab209217).
TLR2 was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, treated with 1 µg/ml LPS for 6 hours, with ab209217 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab209217 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours, 10 µg (Input).
Lane 2: ab209217 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209217 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 6 hours.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)](https://www.abcam.com/ps/products/223/ab223545/Images/ab223545-2-benefits-of-recombinant-antibodies.png)
Anti-TLR2 antibody [EPR20303] - Low endotoxin, Azide free (ab223545)
