Anti-Integrin beta antibody [EPR20825] - BSA and Azide free (ab225742)
![Anti-Integrin beta antibody [EPR20825] - BSA and Azide free (ab225742)](https://www.abcam.com/ps/products/225/ab225742/Images/ab225742-3-ab210515286546210515IHCb.jpg "Anti-Integrin beta antibody [EPR20825] - BSA and Azide free (ab225742)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20825] to Integrin beta - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Integrin beta antibody [EPR20825] - BSA and Azide free
See all Integrin beta primary antibodies -
Description
Rabbit monoclonal [EPR20825] to Integrin beta - BSA and Azide free -
Host species
Rabbit -
Specificity
The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human spleen tissue.
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General notes
Ab225742 is the carrier-free version of ab210515. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab225742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20825 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR20825] - BSA and Azide free (ab225742)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of mouse spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210515).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR20825] - BSA and Azide free (ab225742)](https://www.abcam.com/ps/products/225/ab225742/Images/ab225742-2-ab210515286545210515IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR20825] - BSA and Azide free (ab225742)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of rat spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210515).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR20825] - BSA and Azide free (ab225742)](https://www.abcam.com/ps/products/225/ab225742/Images/ab225742-286550-225742IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR20825] - BSA and Azide free (ab225742)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210515).
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![Anti-Integrin beta antibody [EPR20825] - BSA and Azide free (ab225742)](https://www.abcam.com/ps/products/225/ab225742/Images/ab225742-4-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta antibody [EPR20825] - BSA and Azide free (ab225742)
