All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 3 : Rat spleen lysate
Lane 4 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 5 : Human fetal spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1-3: 3 minutes; Lane 4: 15 seconds; Lane 5: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen (PMID: 22434870). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution or Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500

Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Comparison of staining with an antibody specific to Integrin beta 3 (ab119992).

The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

 

Exposure:

For ab210515: 1, 2, 5 lanes: 3 s; 3, 4 lanes: 37 s; 6 lane: 3 min; 7 lane: 15 s; 8 lane: 114 s. 

For ab119992: 1, 2 lane: 10 s; 3~8 lanes: 3 min

All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : C6 (rat glial tumor cell line) whole cell lysate
Lane 3 : Rat heart lysate
Lane 4 : Rat liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds; Lane 3: 3 minutes; Lane 4: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of mouse spleen (PMID: 22434870). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of rat spleen (PMID: 22434870). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).