Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293)
![Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293)](https://www.abcam.com/ps/products/81/ab81293/Images/ab81293-254771-anti-fak-phospho-y861-antibody-ep841y-western-blot.jpg "Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293)")
Key features and details
- Rabbit monoclonal [EP841Y] to FAK (phospho Y861)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho Y861) antibody [EP841Y]
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP841Y] to FAK (phospho Y861) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human FAK (phospho Y861). The exact sequence is proprietary.
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Positive control
- Hela cell lysates treated with pervanadate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP841Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293)All lanes : Anti-FAK (phospho Y861) antibody [EP841Y] (ab81293) at 1/50000 dilution
Lane 1 : HeLa cell lysates treated with pervanadate
Lane 2 : HeLa cell lysates, untreated
Secondary
All lanes : HRP goat ant-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
