Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 147 417 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP695Y] to FAK
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-FAK antibody [EP695Y]
See all FAK primary antibodies
Description

Rabbit monoclonal [EP695Y] to FAK
Host species

Rabbit
Specificity

ab40794 recognises Focal adhesion kinase (FAK).

The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human FAK aa 700-800. The exact sequence is proprietary.
(Peptide available as ab211922)
Positive control
- WB: NIH/3T3, HeLa, A431, HEK-293T and K-562 cell lysates; Rat tissue lysate. IHC-P: Human hepatocellular carcinoma.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP695Y
Isotype

IgG
Research areas

Western blot - Anti-FAK antibody [EP695Y] (ab40794)

All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : PTK2 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 119 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-FAK antibody [EP695Y] (ab40794)

Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 119 kDa
Western blot - Anti-FAK antibody [EP695Y] (ab40794)

Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 119 kDa
Western blot - Anti-FAK antibody [EP695Y] (ab40794)

Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution + Hela cell lysate

Predicted band size: 119 kDa
Observed band size: 119 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.
See Abreview
Western blot - Anti-FAK antibody [EP695Y] (ab40794) This image is courtesy of an Abreview submitted by Magali Boissiere (6970246)

All lanes : Anti-FAK antibody [EP695Y] (ab40794)

All lanes : Milk PBS Tween

Blocking peptides at 5 % per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit poly clonal at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 119 kDa
Observed band size: 130 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes

Western blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4ºC. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.

See Abreview
Anti-FAK antibody [EP695Y] (ab40794)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com