Anti-FAK (phospho Y925) antibody (ab38512)
Key features and details
- Rabbit polyclonal to FAK (phospho Y925)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho Y925) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho Y925) -
Host species
Rabbit -
Specificity
This antibody is specific for FAK only when phosphorylated at tyrosine 925. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from Human FAK around the phosphorylation site of tyrosine 925 (KVYpEN).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y925) antibody (ab38512)All lanes : Anti-FAK (phospho Y925) antibody (ab38512) at 1/500 dilution
Lane 1 : HepG2 cell lysate, pre-incubated with phosphopeptide.
Lane 2 : HepG2 cell lysate.
Lane 3 : 293 cell lysate + EGF + serum.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L).
Predicted band size: 119 kDa
Observed band size: 123 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein.
