Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)
![Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-307270-anti-fak-phospho-y576-antibody-ep1897y-western-blot.jpg "Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1897Y] to FAK (phospho Y576)
- Suitable for: Dot blot, WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-FAK (phospho Y576) antibody [EP1897Y]
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP1897Y] to FAK (phospho Y576) -
Host species
Rabbit -
Specificity
ab76120 detects FAK phosphorylated at Y576. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman
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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyrosine 576 of human FAK
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Positive control
- HeLa cell lysate and 3T3 cell lysates treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1897Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution (Purified)
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate
Lane 3 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration 5% NFDM/TBST
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![Flow Cytometry - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-307273-anti-fak-phospho-y576-antibody-ep1897y-flow-cytometry.jpg)
Flow Cytometry - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)Flow cytometry analysis of HeLa cells treated with 1mM Pervanadate for 30mins cells labeling FAK (phospho Y576)with ab76120 at 1/400 dilution (0.1µg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 / Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-307272-anti-fak-phospho-y576-antibody-ep1897y-dot-blot.jpg)
Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)Dot blot analysis of FAK (phospho Y576) phospho peptide (Lane 1) and FAK non-phospho peptide (Lane 2) labelling FAK (phospho Y576) phospho peptide with ab76120 at a dilution of 1/1000 dilution (0.441ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
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![Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-307271-anti-fak-phospho-y576-antibody-ep1897y-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration 5% NFDM/TBST
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![Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-91458-anti-fak-phospho-y576-antibody-ep1897y-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/50000 dilution
Lane 1 : 3T3 cell lysates untreated
Lane 2 : 3T3 cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
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![Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)](https://www.abcam.com/ps/products/76/ab76120/Images/ab76120-6-benefits-of-recombinant-antibodies.png)
Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)
