Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
![Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)](https://www.abcam.com/ps/products/276/ab276068/Images/ab276068-2-anti-abhd14b-antibody-ip-jurkat-hela-cells.jpg "Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [011] to ABHD14B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-ABHD14B antibody [011] - BSA and Azide free
See all ABHD14B primary antibodies -
Description
Rabbit monoclonal [011] to ABHD14B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human ABHD14B aa 1-210.
Database link: Q96IU4-1 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat and HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
011 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ABHD14B antibody [011] (ab276068)
ABHD14B was immunoprecipitated from 0.5mg Jurkat (human T cell leukemia cell line from peripheral blood) and HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lane A and B, respectively) with 0.5 µL ab276068 and 15 μl of 50 % Protein G agarose. Western blot was perfoermed from immunoprecipitate using ab276068 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.
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![Western blot - Anti-ABHD14B antibody [011] (ab276068)](https://www.abcam.com/ps/products/276/ab276068/Images/ab276068-1-anti-abhd14b-antibody-wb-jurkat-cells.jpg)
Western blot - Anti-ABHD14B antibody [011] (ab276068)Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 22 kDa
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![Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)](https://www.abcam.com/ps/products/276/ab276068/Images/ab276068-3-benefits-of-recombinant-antibodies.png)
Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
