Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [011] to ABHD14B - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ABHD14B antibody [011] - BSA and Azide free
    See all ABHD14B primary antibodies

  • Description

    Rabbit monoclonal [011] to ABHD14B - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human ABHD14B aa 1-210.
    Database link: Q96IU4-1

  • Positive control

    • WB: Jurkat whole cell lysate. IP: Jurkat and HeLa whole cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    011

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-ABHD14B antibody [011] (ab276068)
    Immunoprecipitation - Anti-ABHD14B antibody [011] (ab276068)

    ABHD14B was immunoprecipitated from 0.5mg Jurkat (human T cell leukemia cell line from peripheral blood) and HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lane A and B, respectively) with 0.5 µL ab276068 and 15 μl of 50 % Protein G agarose. Western blot was perfoermed from immunoprecipitate using ab276068 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution. 

    Developed using the odssey technique.

    Performed under reducing conditions.

  • Western blot - Anti-ABHD14B antibody [011] (ab276068)
    Western blot - Anti-ABHD14B antibody [011] (ab276068)
    Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 22 kDa

  • Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)
    Anti-ABHD14B antibody [011] - BSA and Azide free (ab276068)

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