Anti-PCDH9 antibody [7G3A2] (ab233710)
Key features and details
- Mouse monoclonal [7G3A2] to PCDH9
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PCDH9 antibody [7G3A2] -
Description
Mouse monoclonal [7G3A2] to PCDH9 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB RatHuman -
Immunogen
Recombinant fragment corresponding to Human PCDH9 aa 1-150. Expressed in E.coli.
Database link: Q9HC56 -
Positive control
- WB: Recombinant human PCDH9 (aa24-148) protein; PCDH9 (aa24-148)-IgG-Fc-transfected HEK-293 cell lysate; C6 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7G3A2 -
Isotype
IgG1 -
Research areas
Images
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Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution + Recombinant human PCDH9 (aa24-148) protein
Developed using the ECL technique.
Predicted band size: 136 kDa
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All lanes : Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human PCDH9 (aa24-148)-IgG-Fc-transfected HEK-293 cell lysate
Developed using the ECL technique.
Predicted band size: 136 kDa
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Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution + C6 (rat glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 136 kDa
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCDH9 (green) using ab233710 at 1/50 dilution in ICC/IF. Actin is labeled with AlexaFluor®555-Phalloidin (red). Blue: DRAQ5 DNA dye.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PCDH9 with ab233710 at 1/200 dilution (green) compared with a negative control (red).