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Cell Biology Other Antibodies

Anti-PCDH9 antibody [7G3A2] (ab233710)

Anti-PCDH9 antibody [7G3A2] (ab233710)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [7G3A2] to PCDH9
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PCDH9 antibody [7G3A2]
  • Description

    Mouse monoclonal [7G3A2] to PCDH9
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PCDH9 aa 1-150. Expressed in E.coli.
    Database link: Q9HC56

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human PCDH9 (aa24-148) protein; PCDH9 (aa24-148)-IgG-Fc-transfected HEK-293 cell lysate; C6 cell lysate. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    7G3A2
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution + Recombinant human PCDH9 (aa24-148) protein

    Developed using the ECL technique.

    Predicted band size: 136 kDa

  • Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    All lanes : Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human PCDH9 (aa24-148)-IgG-Fc-transfected HEK-293 cell lysate

    Developed using the ECL technique.

    Predicted band size: 136 kDa

  • Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Western blot - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Anti-PCDH9 antibody [7G3A2] (ab233710) at 1/500 dilution + C6 (rat glioma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 136 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Immunocytochemistry/ Immunofluorescence - Anti-PCDH9 antibody [7G3A2] (ab233710)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCDH9 (green) using ab233710 at 1/50 dilution in ICC/IF. Actin is labeled with AlexaFluor®555-Phalloidin (red). Blue: DRAQ5 DNA dye. 

  • Flow Cytometry - Anti-PCDH9 antibody [7G3A2] (ab233710)
    Flow Cytometry - Anti-PCDH9 antibody [7G3A2] (ab233710)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PCDH9 with ab233710 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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