Anti-FAK (phospho Y861) antibody (ab200811)
Key features and details
- Rabbit polyclonal to FAK (phospho Y861)
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho Y861) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho Y861) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Predicted to work with: Rat, Frog
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Immunogen
Synthetic peptide corresponding to Human FAK (phospho Y861). A chemically synthesized phosphopeptide derived from the region of Human FAK containing tyrosine 861.
Database link: Q05397
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody negatively preadsorbed using a nonphosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y861) antibody (ab200811)All lanes : Anti-FAK (phospho Y861) antibody (ab200811) at 1/1000 dilution
Lane 1 : Extracts of primary CEF cells expressing FAK with phosphopeptide immunogen
Lane 2 : Extracts of primary CEF cells expressing FAK with a generic phosphotyrosine-containing peptide
Lane 3 : Extracts of primary CEF cells expressing FAK with a non-phosphorylated peptide corresponding to the immunogen
Lane 4 : Extracts of primary CEF cells expressing FAK
Secondary
All lanes : Goat F(ab’)2 anti-rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 119 kDaOnly the phosphopeptide corresponding to FAK [pY861]
blocks the antibody signal (lane 1) demonstrating the
specificity of the antibody.
