Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15506(B)] to VTI1B
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-VTI1B antibody [EPR15506(B)]
See all VTI1B primary antibodies
Description

Rabbit monoclonal [EPR15506(B)] to VTI1B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7 and HeLa cell lysates; Human fetal lung and fetal liver lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15506(B)
Isotype

IgG
Research areas

Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : VTI1B knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 28 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab184170 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184170 Anti-VTI1B antibody [EPR15506(B)] was shown to specifically react with VTI1B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265483 (knockout cell lysate ab257797) was used. Wild-type and VTI1B knockout samples were subjected to SDS-PAGE. ab184170 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Flow cytometric analysis of Hela cells (2% paraformaldehyde-fixed) labeling VTI1B with ab184170 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/2000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/10000 dilution + MCF7 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma labeling VTI1B with ab184170 at 1:100 diluton and HRP polymer. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling VTI1B with ab184170 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor^® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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