Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VTI1B antibody [EPR15506(B)] (ab184170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15506(B)] to VTI1B
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-VTI1B antibody [EPR15506(B)]
    See all VTI1B primary antibodies

  • Description

    Rabbit monoclonal [EPR15506(B)] to VTI1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7 and HeLa cell lysates; Human fetal lung and fetal liver lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15506(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : VTI1B knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab184170 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab184170 Anti-VTI1B antibody [EPR15506(B)]  was shown to specifically react with VTI1B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265483 (knockout cell lysate ab257797) was used.  Wild-type and VTI1B knockout samples were subjected to SDS-PAGE.  ab184170 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Flow Cytometry - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Flow cytometric analysis of Hela cells (2% paraformaldehyde-fixed) labeling VTI1B with ab184170 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    All lanes : Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/2000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

  • Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/10000 dilution + MCF7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma labeling VTI1B with ab184170 at 1:100 diluton and HRP polymer.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)

    Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling VTI1B with ab184170 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-VTI1B antibody [EPR15506(B)] (ab184170)
    Anti-VTI1B antibody [EPR15506(B)] (ab184170)

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