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Anti-WNK1 antibody [EPR2743(3)] (ab174854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2743(3)] to WNK1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-WNK1 antibody [EPR2743(3)]
    See all WNK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2743(3)] to WNK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human WNK1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H4A3

  • Positive control

    • 293T and Human fetal brain lysates; 293T cells.

  • General notes

    This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2743(3)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    Western blot - Anti-WNK1 antibody [EPR2743(3)] (ab174854)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: WNK1 knockout HAP1 whole cell lysate (40 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab174854 observed at 300 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab174854 was shown to recognize WNK1 when WNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and WNK1 knockout samples were subjected to SDS-PAGE. Ab174854 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody [EPR2743(3)] (ab174854)

    Immunofluorescent analysis of 293T cells labeling WNK1 with ab174854 at 1/100 dilution (red). Nuclear DAPI staining (blue).

  • Western blot - Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    Western blot - Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    All lanes : Anti-WNK1 antibody [EPR2743(3)] (ab174854) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Human fetal brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 250 kDa

  • Flow Cytometry - Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    Flow Cytometry - Anti-WNK1 antibody [EPR2743(3)] (ab174854)

    Flow cytometric analysis of permeabilized 293T cells labeling WNK1 with ab174854 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

  • Anti-WNK1 antibody [EPR2743(3)] (ab174854)
    Anti-WNK1 antibody [EPR2743(3)] (ab174854)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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