Anti-WNK1 antibody (ab35069)
Key features and details
- Rabbit polyclonal to WNK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WNK1 antibody
See all WNK1 primary antibodies -
Description
Rabbit polyclonal to WNK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 2350 to the C-terminus of Human WNK1.
Read Abcam's proprietary immunogen policy (Peptide available as ab36094.) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/250. Detects a band of approximately 200 kDa (predicted molecular weight: 250 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
1/250. Detects a band of approximately 200 kDa (predicted molecular weight: 250 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. -
Tissue specificity
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron. -
Involvement in disease
Pseudohypoaldosteronism 2C
Neuropathy, hereditary sensory and autonomic, 2A -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsO-glycosylated.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 65125 Human
- Entrez Gene: 232341 Mouse
- Entrez Gene: 116477 Rat
- Omim: 605232 Human
- SwissProt: Q9H4A3 Human
- SwissProt: P83741 Mouse
- SwissProt: Q9JIH7 Rat
- Unigene: 744906 Human
see all -
Alternative names
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
- HSN2 antibody
see all
Images
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Western blot - Anti-WNK1 antibody (ab35069)All lanes : Anti-WNK1 antibody (ab35069) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 250 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.The band observed at 200 kDa could potentially be isoform 4 of the protein which has a predicted molecular weight of 206 kDa (Swiss-Prot data).
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Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody (ab35069)ICC/IF image of ab35069 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab35069, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab35069 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-WNK1 antibody (ab35069)All lanes : Anti-WNK1 antibody (ab35069) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 250 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 55&60 kDa. We are unsure as to the identity of these extra bands.The band observed at 200 kDa could potentially be isoform 4 of the protein which has a predicted molecular weight of 206 kDa (Swiss-Prot data).
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Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody (ab35069)ICC/IF image of ab35069 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab35069, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
