Anti-WNK1 (phospho T58) antibody (ab53137)
Key features and details
- Rabbit polyclonal to WNK1 (phospho T58)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WNK1 (phospho T58) antibody
See all WNK1 primary antibodies -
Description
Rabbit polyclonal to WNK1 (phospho T58) -
Host species
Rabbit -
Specificity
ab53137 detects endogenous levels of WNK1 only when phosphorylated at threonine 58. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WNK1 (phospho T58) antibody (ab53137)All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution
Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 251 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK1 (phospho T58) antibody (ab53137)ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
