Anti-DCK antibody [OTI16E12] (ab118994)
![Anti-DCK antibody [OTI16E12] (ab118994)](https://www.abcam.com/ps/products/118/ab118994/Images/ab118994-131647-anti-dck-antibody-oti16e12-western-blot.jpg "Anti-DCK antibody [OTI16E12] (ab118994)")
Key features and details
- Mouse monoclonal [OTI16E12] to DCK
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DCK antibody [OTI16E12]
See all DCK primary antibodies -
Description
Mouse monoclonal [OTI16E12] to DCK -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human DCK aa 1-300. (NP_000779) produced in HEK293T cells.
Database link: P27707 -
Positive control
- WB: HEK293T cells transfected with pCMV6-ENTRY DCK. Flow Cyt (Intra): HEK-293T transfected with DCK, HeLa and Jurkat cells.
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General notes
The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI16E12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DCK antibody [OTI16E12] (ab118994)All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa
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![Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)](https://www.abcam.com/ps/products/118/ab118994/Images/ab118994-131646-anti-dck-antibody-oti16e12-flow-cytometry.jpg)
Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.
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![Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)](https://www.abcam.com/ps/products/118/ab118994/Images/ab118994-131645-anti-dck-antibody-oti16e12-flow-cytometry.jpg)
Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).
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![Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)](https://www.abcam.com/ps/products/118/ab118994/Images/ab118994-131644-anti-dck-antibody-oti16e12-flow-cytometry.jpg)
Flow Cytometry - Anti-DCK antibody [OTI16E12] (ab118994)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
