Anti-Dityrosine antibody [10A6] (ab243067)
![Anti-Dityrosine antibody [10A6] (ab243067)](https://www.abcam.com/ps/products/243/ab243067/Images/ab243067-332296-67b.png "Anti-Dityrosine antibody [10A6] (ab243067)")
Key features and details
- Mouse monoclonal [10A6] to Dityrosine
- Suitable for: Flow Cyt, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-Dityrosine antibody [10A6] -
Description
Mouse monoclonal [10A6] to Dityrosine -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Dityrosine conjugated to keyhole limpet haemocyanin.
-
Positive control
- WB: Dityrosine-BSA conjugate; HeLa cell lysate (+/- hydrogen peroxide treatment). Flow cyt: SH-SY5Y cells (+/- hydrogen peroxide treatment).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
10A6 -
Isotype
IgG1
Images
-
Western blot - Anti-Dityrosine antibody [10A6] (ab243067)All lanes : Anti-Dityrosine antibody [10A6] (ab243067) at 1/1000 dilution
Lane 1 : BSA
Lane 2 : 3,5-Dibromotyrosine-BSA
Lane 3 : Dityrosine-BSA
Lane 4 : Bromotyrosine-BSA
Lane 5 : 7-ketocholesterol-BSA
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Synthetic Dityrosine conjugated to Keyhole Limpet Kemocyanin (KLH). at 1/20000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
-
![Western blot - Anti-Dityrosine antibody [10A6] (ab243067)](https://www.abcam.com/ps/products/243/ab243067/Images/ab243067-332297-67d.png)
Western blot - Anti-Dityrosine antibody [10A6] (ab243067)All lanes : Anti-Dityrosine antibody [10A6] (ab243067) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (treated with hydrogen peroxide) cell lysate
Lysates/proteins at 12 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
-
![Flow Cytometry - Anti-Dityrosine antibody [10A6] (ab243067)](https://www.abcam.com/ps/products/243/ab243067/Images/ab243067-332298-67c.png)
Flow Cytometry - Anti-Dityrosine antibody [10A6] (ab243067)Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+/- 250 μM hydrogen peroxide treatment for 24 hours) labeling Dityrosine with ab243067 at 1/50 dilution for 30 minutes on ice compared with a Isotype control IgG and an unlabeled control. A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
