Anti-VASP (phospho S157) antibody [5C6] (ab58555)
![Anti-VASP (phospho S157) antibody [5C6] (ab58555)](https://www.abcam.com/ps/products/58/ab58555/Images/ab58555-72443-anti-vasp-phospho-s157-antibody-5c6-flow-cytometry.jpg "Anti-VASP (phospho S157) antibody [5C6] (ab58555)")
Key features and details
- Mouse monoclonal [5C6] to VASP (phospho S157)
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-VASP (phospho S157) antibody [5C6]
See all VASP primary antibodies -
Description
Mouse monoclonal [5C6] to VASP (phospho S157) -
Host species
Mouse -
Specificity
This antibody recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. It does not cross-react with non-phosphorylated VASP or with un-related phosphorylation sites. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic phosphopeptide corresponding to amino acids surrounding the Ser157 phosphorylation site of human VASP
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Positive control
- Vanadate-treated A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. -
Clonality
Monoclonal -
Clone number
5C6 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (ab58555)Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
