Antibodies, Proteins, Kits and Reagents for Life Science

Anti-VASP (phospho S239) antibody [EPR21734] - BSA and Azide free (ab242021)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21734] to VASP (phospho S239) - BSA and Azide free
Suitable for: WB, Dot blot, IP
Reacts with: Human

Product name

Anti-VASP (phospho S239) antibody [EPR21734] - BSA and Azide free
See all VASP primary antibodies
Description

Rabbit monoclonal [EPR21734] to VASP (phospho S239) - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IP: THP-1 treated with 10uM forskolin for 30 minutes whole cell lysate.
General notes
Ab242021 is the carrier-free version of ab218619. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242021 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21734
Isotype

IgG
Research areas

Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] - BSA and Azide free (ab242021)

VASP (phospho S239) was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia cell line) treated with 10µM forskolin for 30min whole cell lysate using ab218619 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab218619 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1: THP-1 treated with 10µM forskolin for 30min whole cell lysate 10μg (input).
Lane 2: ab218619 IP in THP-1 treated with 10µM forskolin for 30min whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218619 in THP-1 treated with 10µM forskolin for 30min whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218619).
Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] - BSA and Azide free (ab242021)

Dot blot analysis of VASP (phospho S239) using ab218619 at 1/1000 dilution, followed byGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1: VASP (phospho S239) peptide
Lane 2: VASP non-phospho peptide.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 58 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218619).
Anti-VASP (phospho S239) antibody [EPR21734] - BSA and Azide free (ab242021)

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