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Anti-VASP antibody [OTI1H8] (ab114029)

Key features and details

  • Mouse monoclonal [OTI1H8] to VASP
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Dog, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-VASP antibody [OTI1H8]
    See all VASP primary antibodies

  • Description

    Mouse monoclonal [OTI1H8] to VASP

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Dog, Human, African green monkey

  • Immunogen

    Recombinant full length protein corresponding to Human VASP aa 1 to the C-terminus. Produced in HEK-293T cells. NP_003361
    Database link: P50552

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA; HepG2, HeLa, MCF7, HT-29 and A549 cell extracts; ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY VASP; Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY VASP overexpress plasmid.

  • General notes

    The clone number has been updated from 1H8 to OTI1H8, both clone numbers name the same clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography

  • Clonality

    Monoclonal

  • Clone number

    OTI1H8

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-VASP antibody [OTI1H8] (ab114029)
    Western blot - Anti-VASP antibody [OTI1H8] (ab114029)
    All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
    Lane 4 : A549 (Human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (Canine kidney cell line) cell extract
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (ab114029)
    Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (ab114029)

    ab114029 at 1/100 dilution staining VASP in COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY VASP by Immunofluorescence.

  • Flow Cytometry - Anti-VASP antibody [OTI1H8] (ab114029)
    Flow Cytometry - Anti-VASP antibody [OTI1H8] (ab114029)

    ab114029 at 1/100 dilution staining VASP in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY VASP overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.

  • Western blot - Anti-VASP antibody [OTI1H8] (ab114029)
    Western blot - Anti-VASP antibody [OTI1H8] (ab114029)
    All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 40 kDa

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