Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1H8] to VASP
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-VASP antibody [OTI1H8]
See all VASP primary antibodies
Description

Mouse monoclonal [OTI1H8] to VASP
Host species

Mouse
Tested applications

Suitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Dog, Human, African green monkey
Immunogen

Recombinant full length protein corresponding to Human VASP aa 1 to the C-terminus. Produced in HEK-293T cells. NP_003361
Database link: P50552

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA; HepG2, HeLa, MCF7, HT-29 and A549 cell extracts; ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY VASP; Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY VASP overexpress plasmid.
General notes

The clone number has been updated from 1H8 to OTI1H8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI1H8
Isotype

IgG1
Research areas

Western blot - Anti-VASP antibody [OTI1H8] (ab114029)

All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (ab114029)

ab114029 at 1/100 dilution staining VASP in COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY VASP by Immunofluorescence.
Flow Cytometry - Anti-VASP antibody [OTI1H8] (ab114029)

ab114029 at 1/100 dilution staining VASP in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY VASP overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
Western blot - Anti-VASP antibody [OTI1H8] (ab114029)

All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 40 kDa

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