Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Wednesday March 10, 2021

Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21734] to VASP (phospho S239)
Suitable for: WB, Dot blot, IP
Reacts with: Human

Product name

Anti-VASP (phospho S239) antibody [EPR21734]
See all VASP primary antibodies
Description

Rabbit monoclonal [EPR21734] to VASP (phospho S239)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1, untreated and treated with 10µM forskolin for 30 minutes, whole cell lysate. IP: THP-1 treated with 10uM forskolin for 30 minutes whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21734
Isotype

IgG
Research areas

Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)

All lanes : Anti-VASP (phospho S239) antibody [EPR21734] (ab218619) at 1/1000 dilution

Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 treated with 10µM forskolin for 30 minutes, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.
Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)

VASP (phospho S239) was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia cell line) treated with 10µM forskolin for 30min whole cell lysate using ab218619 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab218619 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1: THP-1 treated with 10µM forskolin for 30min whole cell lysate 10μg (input).
Lane 2: ab218619 IP in THP-1 treated with 10µM forskolin for 30min whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218619 in THP-1 treated with 10µM forskolin for 30min whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)

Dot blot analysis of VASP (phospho S239) using ab218619 at 1/1000 dilution, followed byGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1: VASP (phospho S239) peptide
Lane 2: VASP non-phospho peptide.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 58 seconds.
Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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