Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12451] to EDIL3/DEL1
Suitable for: ICC/IF, WB, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-EDIL3/DEL1 antibody [EPR12451]
See all EDIL3/DEL1 primary antibodies
Description

Rabbit monoclonal [EPR12451] to EDIL3/DEL1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HUVEC, BxPC-3, 293, C6, Raw264.7, PC12 and NIH 3T3 cell lysate; A431 and HeLa cells
General notes
Previously labelled as EDIL3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12451
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

Immunofluorescent staining of 4% paraformaldehyde fixed Hela cells labeling EDIL3/DEL1 with ab190692 at 1/250 dilution (green) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor^® 488). The image also shows negative control cells.
Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

All lanes : Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa
Western blot - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

All lanes : Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa
Flow Cytometry - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3/DEL1 with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).
Immunoprecipitation - Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3/DEL1 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.
Anti-EDIL3/DEL1 antibody [EPR12451] (ab190692)

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