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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Endothelial

Anti-CD151 antibody [B-E31] - BSA and Azide free (ab269712)

Anti-CD151 antibody [B-E31] - BSA and Azide free (ab269712)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [B-E31] to CD151 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD151 antibody [B-E31] - BSA and Azide free
    See all CD151 primary antibodies
  • Description

    Mouse monoclonal [B-E31] to CD151 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    This antibody is specific for CD151.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to CD151.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    ab269712 is a carrier free version of ab27406. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    This antibody is sterile filtered through .22 µm and treated to remove endotoxins. It is carrier and preservative free.
  • Clonality

    Monoclonal
  • Clone number

    B-E31
  • Myeloma

    P3x63-Ag8.653
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody (ab269712)
    Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody (ab269712)

    ICC/IF image of ab27406 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27406, 10µg/ml) overnight at +4°C then with the secondary antibody (green) for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27406).

  • Flow Cytometry - Anti-CD151 antibody [B-E31] - BSA and Azide free (ab269712)
    Flow Cytometry - Anti-CD151 antibody [B-E31] - BSA and Azide free (ab269712)

    Ab27406, staining CD151 by FACS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27406).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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