Anti-VASP antibody (ab229624)
Key features and details
- Rabbit polyclonal to VASP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-VASP antibody
See all VASP primary antibodies -
Description
Rabbit polyclonal to VASP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VASP (internal sequence). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P50552 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: MDA-MB-231 xenograft tissue. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-VASP antibody (ab229624)All lanes : Anti-VASP antibody (ab229624) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
-
Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody (ab229624)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for VASP (green) using ab229624 at a 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 10 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP antibody (ab229624)Paraffin-embedded MDA-MB-231 xenograft stained for VASP with ab229624 at 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-VASP antibody (ab229624)All lanes : Anti-VASP antibody (ab229624) at 1/2000 dilution
Lane 1 : Untreated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Forskolin-treated (10 µM, 20 minutes) HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
