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Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1337(2)] to VASP - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-VASP antibody [EPR1337(2)] - BSA and Azide free
    See all VASP primary antibodies

  • Description

    Rabbit monoclonal [EPR1337(2)] to VASP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HEK293, 293T, HepG2, THP-1 and human platelet lysates. IP: HEK293 cell lysate.

  • General notes

    Ab231823 is the carrier-free version of ab109321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231823 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)
    Western blot - Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)
    All lanes : Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : VASP knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 40 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109321, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab109321 observed at 46 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab109321 Anti-VASP antibody [EPR1337(2)] was shown to specifically react with VASP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265892 (knockout cell lysate ab257792) was used. Wild-type and VASP knockout samples were subjected to SDS-PAGE. ab109321 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)
    Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)

    ab109321 (purified) at 1/30 immunoprecipitating VASP in HEK293 cell lysate (Lane 1). Lane 2 - rabbit monoclonal IgG instead of ab109321 in HEK293 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109321).

  • Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)
    Anti-VASP antibody [EPR1337(2)] - BSA and Azide free (ab231823)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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