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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions

Anti-VASP antibody [EPR1337(2)] (ab109321)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-VASP antibody [EPR1337(2)] (ab109321)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1337(2)] to VASP
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-VASP antibody [EPR1337(2)]
    See all VASP primary antibodies
  • Description

    Rabbit monoclonal [EPR1337(2)] to VASP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HEK293, 293T, HepG2, THP-1 and human platelet lysates. IP: HEK293 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1337(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions

Images

  • Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    All lanes : Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : VASP knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 40 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab109321 observed at 46 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab109321 Anti-VASP antibody [EPR1337(2)] was shown to specifically react with VASP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265892 (knockout cell lysate ab257792) was used. Wild-type and VASP knockout samples were subjected to SDS-PAGE. ab109321 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 40 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 40 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/5000 dilution (purified) + THP-1 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 40 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321)
    All lanes : Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/10000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (ab109321)
    Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (ab109321)

    ab109321 (purified) at 1/30 immunoprecipitating VASP in HEK293 cell lysate (Lane 1). Lane 2 - rabbit monoclonal IgG instead of ab109321 in HEK293 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-VASP antibody [EPR1337(2)] (ab109321)
    Anti-VASP antibody [EPR1337(2)] (ab109321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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