Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free
    See all Paxillin primary antibodies

  • Description

    Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects Paxillin phosphorylated on Tyrosine 118.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247906 is the carrier-free version of ab109547. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247906 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate
    Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    This data was developed using ab109547, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    This data was developed using ab109547, the same antibody clone in a different buffer formulation.Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/BST. Dilution buffer: 5% NFDM /TBST.
  • Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 65 kDa



    This data was developed using ab109547, the same antibody clone in a different buffer formulation.

     

  • Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)
    Anti-Paxillin (phospho Y118) antibody [EPR1903] - BSA and Azide free (ab247906)

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