Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22515-75] to Reelin - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Reelin antibody [EPR22515-75] - BSA and Azide free
    See all Reelin primary antibodies

  • Description

    Rabbit monoclonal [EPR22515-75] to Reelin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse hippocampus tissue. Mouse, human and rat cerebral cortex tissue.

  • General notes

    ab259267 is the carrier-free version of ab230820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259267 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259267).

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259267).

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons of mouse cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259267).

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons of human cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259267).

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons rat cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259267).

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Negative control: No staining on human placenta.

    The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)
    Anti-Reelin antibody [EPR22515-75] - BSA and Azide free (ab259267)

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