Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20822] to BHMT + BHMT2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR20822] to BHMT + BHMT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    Ab230151 is the carrier-free version of ab213491. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230151 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat liver is observed (PMID: 9281325; PMID: 26592251).counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213491).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BHMT + BHMT2 with ab213491 at a 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human liver is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213491).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human kidney tissue is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It’s mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

    Note: It can show nuclear staining if working at higher concentration.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide  (ab213491).

  • Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)
    Anti-BHMT + BHMT2 antibody [EPR20822] - BSA and Azide free (ab230151)

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