Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAH antibody [EPR12381] - C-terminal (ab191415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12381] to PAH - C-terminal
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAH antibody [EPR12381] - C-terminal
    See all PAH primary antibodies

  • Description

    Rabbit monoclonal [EPR12381] to PAH - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293T, HepG2, RAW 264.7, C6, NIH 3T3, PC12, Human fetal liver and U87-MG whole cell lysatse; Human liver and Mouse kidney tissues; U87-MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Western blot - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    All lanes : Anti-PAH antibody [EPR12381] - C-terminal (ab191415) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Flow Cytometry - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Flow Cytometry - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)

    Flow cytometric analysis of U87-MG cells (paraformaldehyde-fixed, 2%) labeling PAH with ab191415 at 1/300 dilution (red), or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PAH with ab191415 at 1/800 dilution (1.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Western blot - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    All lanes : Anti-PAH antibody [EPR12381] - C-terminal (ab191415) at 1/10000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12381] - C-terminal (ab191415)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PAH with ab191415 at 1/800 dilution (1.9 μg/ml)  followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PAH antibody [EPR12381] - C-terminal (ab191415)
    Anti-PAH antibody [EPR12381] - C-terminal (ab191415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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