Anti-PAH antibody (ab232956)
Key features and details
- Rabbit polyclonal to PAH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-PAH antibody
See all PAH primary antibodies -
Description
Rabbit polyclonal to PAH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment (His-tag) corresponding to Human PAH aa 200 to the C-terminus. Expressed in E.coli. N-terminal tag.
Database link: P00439 -
Positive control
- WB: Recombinant human PAH protein; Human serum; Human liver lysate; Pig kidney lysate; Mouse gallbladder lysate. IHC-P: Human liver, liver cancer, breast cancer and kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAH antibody (ab232956)All lanes : Anti-PAH antibody (ab232956) at 2 µg
Lane 1 : Human serum
Lane 2 : Human liver lysate
Lane 3 : Pig kidney lysate
Lane 4 : Mouse gallbladder lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody (ab232956)Formalin-fixed, paraffin-embedded human liver tissue stained for PAH using ab232956 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody (ab232956)Formalin-fixed, paraffin-embedded human liver cancer tissue stained for PAH using ab232956 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody (ab232956)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PAH using ab232956 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody (ab232956)Formalin-fixed, paraffin-embedded human kidney tissue stained for PAH using ab232956 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-PAH antibody (ab232956)Anti-PAH antibody (ab232956) at 2 µg + Recombinant human PAH protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
