Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9760(B)] to PCBD1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-PCBD1 antibody [EPR9760(B)]
See all PCBD1 primary antibodies
Description

Rabbit monoclonal [EPR9760(B)] to PCBD1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PCBD1 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Human ovary cancer, fetal heart, fetal liver, HEK293T and Caco-2 cell lysates. IHC-P: Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9760(B)
Isotype

IgG
Research areas

Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/500 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PCBD1 knockout HEK293T cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human ovary cancer tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 12 kDa
Observed band size: 12 kDa

Lanes 1-4: Merged signal (red and green). Green - ab138518 observed at 12 kDa. Red - loading control ab8245 observed at 36 kDa.

ab138518 Anti-PCBD1 antibody [EPR9760(B)] was shown to specifically react with PCBD1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266261 (knockout cell lysate ab258095) was used. Wild-type and PCBD1 knockout samples were subjected to SDS-PAGE. ab138518 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling PCBD1 with ab138518 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/1000 dilution

Lane 1 : Human ovary
cancer cell lysate
Lane 2 : Fetal heart cell lysate
Lane 3 : Fetal liver cell lysate
Lane 4 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa
Observed band size: 12 kDa
Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com