Anti-CBL (phospho Y774) antibody [E160] (ab32446)
![Anti-CBL (phospho Y774) antibody [E160] (ab32446)](https://www.abcam.com/ps/products/32/ab32446/Images/ab32446-44881-anti-cbl-phospho-y774-antibody-e160-western-blot.jpg "Anti-CBL (phospho Y774) antibody [E160] (ab32446)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E160] to CBL (phospho Y774)
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CBL (phospho Y774) antibody [E160]
See all CBL primary antibodies -
Description
Rabbit monoclonal [E160] to CBL (phospho Y774) -
Host species
Rabbit -
Specificity
The antibody detects Cbl phosphorylated on Tyrosine 774. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human Cbl.
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Positive control
- Jurkat whole cell lysate (ab7899) and human cervical carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
E160 -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-CBL (phospho Y774) antibody [E160] (ab32446)All lanes : Anti-CBL (phospho Y774) antibody [E160] (ab32446) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate + Pervanadate
Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] (ab32446)](https://www.abcam.com/ps/products/32/ab32446/Images/ab32446-44880-anti-cbl-phospho-y774-antibody-e160-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] (ab32446)Immunohistochemical analysis of human cervical carcinoma using ab32446 at 1/25 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] (ab32446)](https://www.abcam.com/ps/products/32/ab32446/Images/ab32446-44879-anti-cbl-phospho-y774-antibody-e160-flow-cytometry.gif)
Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] (ab32446)Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or Pervanadate-treated (red), using ab32446 at 1/20 dilution. -
![Anti-CBL (phospho Y774) antibody [E160] (ab32446)](https://www.abcam.com/ps/products/32/ab32446/Images/ab32446-4-benefits-of-recombinant-antibodies.png)
Anti-CBL (phospho Y774) antibody [E160] (ab32446)
