Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
![Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)](https://www.abcam.com/ps/products/76/ab76536/Images/ab76536-92009-anti-cbl-phospho-y674-antibody-epr2227-western-blot.jpg "Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2227] to CBL (phospho Y674)
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CBL (phospho Y674) antibody [EPR2227]
See all CBL primary antibodies -
Description
Rabbit monoclonal [EPR2227] to CBL (phospho Y674) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding tyrosine 674 of human CBL.
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Positive control
- Jurkat cell lysate treated with pervanadate. Permeabilized Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2227 -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)All lanes : Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536) at 1/1000 dilution
Lane 1 : Jurkat cell
lysate
Lane 2 : Jurkat cell
lysate treated with
pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 100 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)](https://www.abcam.com/ps/products/76/ab76536/Images/ab76536-92008-anti-cbl-phospho-y674-antibody-epr2227-flow-cytometry.jpg)
Flow Cytometry - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)Flow cytometric analysis of permeabilized Jurkat cells, un-treated (green) or pervanadate treated (red) using ab76536 at a 1/20 dilution, and pervanadate-treated Jurkat cells using the same antibody pre-incubated with phospho-CBL peptide (blue) or non-phospho-CBL peptide (orange). -
![Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)](https://www.abcam.com/ps/products/76/ab76536/Images/ab76536-3-benefits-of-recombinant-antibodies.png)
Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
