Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)
![Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)](https://www.abcam.com/ps/products/180/ab180188/Images/ab180188-2-anti-vglut1-antibody-epr10953-c-terminal-immunocytochemistry-mouse-primary-neuron-cell8.jpg "Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10953] to VGluT1 - C-terminal
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VGluT1 antibody [EPR10953] - C-terminal
See all VGluT1 primary antibodies -
Description
Rabbit monoclonal [EPR10953] to VGluT1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VGluT1 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9P2U7 -
Positive control
- WB: Fetal brain, Neuro-2a and C6 lysates; ICC: Mouse primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10953 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling VGluT1 with ab180188 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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![Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)](https://www.abcam.com/ps/products/180/ab180188/Images/ab180188-208827-anti-vglut1-antibody-epr10953-c-terminal-western-blot.jpg)
Western blot - Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)All lanes : Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Neuro-2a lysate
Lane 3 : C6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
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![Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)](https://www.abcam.com/ps/products/180/ab180188/Images/ab180188-3-benefits-of-recombinant-antibodies.png)
Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188)
