Anti-VGluT1 antibody [N28-9] (ab134283)
![Anti-VGluT1 antibody [N28-9] (ab134283)](https://www.abcam.com/ps/products/134/ab134283/Images/ab134283-148627-anti-vglut1-antibody-n28-9-western-blot.jpg "Anti-VGluT1 antibody [N28-9] (ab134283)")
Key features and details
- Mouse monoclonal [N28-9] to VGluT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-VGluT1 antibody [N28-9]
See all VGluT1 primary antibodies -
Description
Mouse monoclonal [N28-9] to VGluT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB MouseRatHuman
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Immunogen
Fusion protein corresponding to Rat VGluT1 aa 450-600 (C terminal).
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Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Brain tissue lysates in Western Blot, and on a formalin-fixed, paraffin-embedded Rat Brain Tissue section in IHC.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N28-9 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-VGluT1 antibody [N28-9] (ab134283)All lanes : Anti-VGluT1 antibody [N28-9] (ab134283) at 5 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 50,55 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134283 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
![Western blot - Anti-VGluT1 antibody [N28-9] (ab134283)](https://www.abcam.com/ps/products/134/ab134283/Images/ab134283-314053-anti-vglut1-antibody-n28-9-western-blot.jpg)
Western blot - Anti-VGluT1 antibody [N28-9] (ab134283)All lanes : Anti-VGluT1 antibody [N28-9] (ab134283) at 5 µg
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Rat Brain Tissue Lysate
Lane 3 : Mouse Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 50,55 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134283 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [N28-9] (ab134283)](https://www.abcam.com/ps/products/134/ab134283/Images/ab134283-148628-anti-vglut1-antibody-n28-9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody [N28-9] (ab134283)IHC image of VGluT1 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
