Anti-VGluT1 antibody (ab77822)
Key features and details
- Rabbit polyclonal to VGluT1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-VGluT1 antibody
See all VGluT1 primary antibodies -
Description
Rabbit polyclonal to VGluT1 -
Host species
Rabbit -
Specificity
Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Pigeon, Human, Zebrafish
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Immunogen
Synthetic peptide corresponding to Rat VGluT1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93078)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab77822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa). Target
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Function
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
Tissue specificity
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. -
Sequence similarities
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 518849 Cow
- Entrez Gene: 57030 Human
- Entrez Gene: 72961 Mouse
- Entrez Gene: 116638 Rat
- Omim: 605208 Human
- SwissProt: A4FV52 Cow
- SwissProt: Q9P2U7 Human
- SwissProt: Q3TXX4 Mouse
see all -
Alternative names
- BNPI antibody
- Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
- Brain specific Na dependent inorganic phosphate cotransporter antibody
see all
Images
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Western blot - Anti-VGluT1 antibody (ab77822)All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Cerebellum Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
References (11)
ab77822 has been referenced in 11 publications.
- Mahoney R et al. Pathogenic Tau Causes a Toxic Depletion of Nuclear Calcium. Cell Rep 32:107900 (2020). PubMed: 32668249
- Siano G et al. Tau Modulates VGluT1 Expression. J Mol Biol 431:873-884 (2019). PubMed: 30664870
- Adamo AM et al. Early Developmental Marginal Zinc Deficiency Affects Neurogenesis Decreasing Neuronal Number and Altering Neuronal Specification in the Adult Rat Brain. Front Cell Neurosci 13:62 (2019). PubMed: 30890920
- Mun SK et al. Losartan Prevents Maladaptive Auditory-Somatosensory Plasticity After Hearing Loss via Transforming Growth Factor-ß Signaling Suppression. Clin Exp Otorhinolaryngol 12:33-39 (2019). PubMed: 30021416
- Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). PubMed: 31368502
Images
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Western blot - Anti-VGluT1 antibody (ab77822)All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Cerebellum Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
