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Neuroscience Cell Adhesion Proteins Membrane Proteins

Anti-M Cadherin antibody [EP5756] (ab129078)

Price and availability

227 827 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-M Cadherin antibody [EP5756] (ab129078)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP5756] to M Cadherin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-M Cadherin antibody [EP5756]
    See all M Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EP5756] to M Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human M Cadherin aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: A673, Human cerebellum, Fetal heart, Fetal brain and 293T cell lysates; Mouse brain, heart, kidney and spleen tissues; Rat brain, kidney and spleen tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP5756
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Images

  • Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml

    Lane 1 : Rat Brain Tissue
    Lane 2 : Rat Heart Tissue
    Lane 3 : Rat Kidney Tissue
    Lane 4 : Rat Spleen Tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 89 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST

  • Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 0.089 µg/ml

    Lane 1 : Mouse Brain Tissue
    Lane 2 : Mouse Heart Tissue
    Lane 3 : Mouse Kidney Tissue
    Lane 4 : Mouse Spleen Tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 89 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST 

  • Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    Western blot - Anti-M Cadherin antibody [EP5756] (ab129078)
    All lanes : Anti-M Cadherin antibody [EP5756] (ab129078) at 1/1000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : Human cerebellum cell lysate
    Lane 3 : Human fetal heart cell lysate
    Lane 4 : Human fetal brain cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP

    Predicted band size: 89 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-M Cadherin antibody [EP5756] (ab129078)

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    Applications: IHC-P

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    Anti-M Cadherin antibody (ab87374)

    Applications: ICC/IF, WB

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