Anti-NF2 / Merlin (phospho S518) antibody (ab131473)
Key features and details
- Rabbit polyclonal to NF2 / Merlin (phospho S518)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF2 / Merlin (phospho S518) antibody
See all NF2 / Merlin primary antibodies -
Description
Rabbit polyclonal to NF2 / Merlin (phospho S518) -
Host species
Rabbit -
Specificity
ab131473 detects endogenous levels of NF2 / Merlin only when phosphorylated at serine 518. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to a region around the phosphorylation site of serine 518 (
RLS(p)ME
) of Human NF2/ Merlin (NP_000259.1). -
Positive control
- HeLa cells; HUVEC cell extract treated with IFN alpha.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131473 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab131473)All lanes : Anti-NF2 / Merlin (phospho S518) antibody (ab131473) at 1/500 dilution
Lane 1 : HUVEC cell extract
Lane 2 : HUVEC cell extract treated with IFN alpha
Predicted band size: 70 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-NF2 / Merlin (phospho S518) antibody (ab131473)Immunofluorescent analysis of methanol-fixed HeLa cells labelling NF2 / Merlin (phospho S518) with ab131473 at 1/100 dilution.
