Anti-NF2 / Merlin antibody (ab238520)
Key features and details
- Rabbit polyclonal to NF2 / Merlin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF2 / Merlin antibody
See all NF2 / Merlin primary antibodies -
Description
Rabbit polyclonal to NF2 / Merlin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Fusion protein corresponding to Human NF2/ Merlin aa 1-600. NP_000259.1
Database link: P35240 -
Positive control
- WB: HeLa, HEK-293T and HepG2 whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NF2 / Merlin antibody (ab238520)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NF2 / Merlin (Green) using ab238520 at 1/20 dilution in ICC/IF. Nuclear counterstain is DAPI (Blue).
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Western blot - Anti-NF2 / Merlin antibody (ab238520)All lanes : Anti-NF2 / Merlin antibody (ab238520) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 70 kDaBlocking buffer: 3% non-fat dry milk in TBST.
