Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)
![Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)](https://www.abcam.com/ps/products/109/ab109244/Images/ab109244-450419-anti-nf2-merlin-antibody-epr25732-western-blot-wildtype-HeLa-nf2-knockout-HeLa.jpg "Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2573(2)] to NF2 / Merlin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-NF2 / Merlin antibody [EPR2573(2)]
See all NF2 / Merlin primary antibodies -
Description
Rabbit monoclonal [EPR2573(2)] to NF2 / Merlin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2573(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)All lanes : Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NF2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab109244 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab109244 was shown to react with NF2 in wild-type HeLa cells in western blot with loss of signal observed in NF2 knockout cell line ab261796 (NF2 knockout cell lysate ab257179). Wild-type and NF2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab109244 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)](https://www.abcam.com/ps/products/109/ab109244/Images/ab109244-286154-anti-nf2-merlin-antibody-epr25732-western-blot.jpg)
Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: NF2 knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab109244 observed at 70 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab109244 was shown to specifically react with NF2 in wild type cells as signal was lost in NF2 knockout cells. Wild-type and NF2 knockout samples were subjected to SDS-PAGE. Ab109244 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)](https://www.abcam.com/ps/products/109/ab109244/Images/ab109244-121930-anti-nf2-merlin-antibody-epr25732-western-blot.jpg)
Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)All lanes : Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
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![Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)](https://www.abcam.com/ps/products/109/ab109244/Images/ab109244-4-benefits-of-recombinant-antibodies.png)
Anti-NF2 / Merlin antibody [EPR2573(2)] (ab109244)
