Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [MBP2] to Myelin Basic Protein - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free
    See all Myelin Basic Protein primary antibodies

  • Description

    Mouse monoclonal [MBP2] to Myelin Basic Protein - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate. IHC-Fr: Mouse cerebrum tissue. IHC-P: Human, mouse and rat cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab277126 is the carrier-free version of ab11159. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Western blot - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Anti-Myelin Basic Protein antibody [MBP2] (ab11159) at 1/1000 dilution + human brain tissue at 20 µg

    Secondary
    Anti-mouse IgG for IP (HRP) (ab131368) at 1/50000 dilution

    Predicted band size: 33 kDa


    Exposure time: 26 seconds


    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rat cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10 (112.5 ug/ml) dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the mouse cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/1000 (1.125 ug/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the human cerebrum. The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

    This data was developed using ab11159 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myelin Basic Protein with ab11159 at 1:1000 (1.125 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining on human spleen (PMID: 9518636). The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)
    Anti-Myelin Basic Protein antibody [MBP2] - BSA and Azide free (ab277126)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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