Anti-NF2 / Merlin antibody (ab245329)
Key features and details
- Rabbit polyclonal to NF2 / Merlin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NF2 / Merlin antibody
See all NF2 / Merlin primary antibodies -
Description
Rabbit polyclonal to NF2 / Merlin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NF2/ Merlin aa 550-590. The exact sequence is proprietary. (NP_057502.2). This epitope is common to NF2/ Merlin isoforms 2, 5 (NP_861966.1), 6 (NP_861967.1) and 7 (NP_861968.1).
Database link: P35240-3 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245329 was affinity purified using an epitope specific to NF2 / Merlin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF2 / Merlin antibody (ab245329)All lanes : Anti-NF2 / Merlin antibody (ab245329) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-NF2 / Merlin antibody (ab245329)NF2 / Merlin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245329 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab245329 at 1 µg/ml.
Lane 1: ab245329 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
