Anti-NF2 / Merlin (phospho S518) antibody (ab2478)
Key features and details
- Rabbit polyclonal to NF2 / Merlin (phospho S518)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NF2 / Merlin (phospho S518) antibody
See all NF2 / Merlin primary antibodies -
Description
Rabbit polyclonal to NF2 / Merlin (phospho S518) -
Host species
Rabbit -
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated Ser 518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross reactivity with NF2 (Merlin) occurs in mouse tissue. Reactivity with NF2 from other sources has not been determined. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Human NF2/ Merlin phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - TDMKRL(pS)MEIEK (aa 508-530).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab2478)
Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot.
Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.
Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot.
Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (ab2478)IHC image of ab2478 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
