Anti-Strumpellin antibody (ab101222)
Key features and details
- Rabbit polyclonal to Strumpellin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Strumpellin antibody
See all Strumpellin primary antibodies -
Description
Rabbit polyclonal to Strumpellin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Zebrafish, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody can a positive signal in the following lysates: Mouse Brain Tissue; Mouse Spinal Cord Tissue; Mouse Lung Tissue; HeLa Whole Cell; HepG2 Whole Cell.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGAssociated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab101222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). Target
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Function
Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. -
Tissue specificity
Expressed ubiquitously. -
Involvement in disease
Defects in KIAA0196 are the cause of spastic paraplegia autosomal dominant type 8 (SPG8) [MIM:603563]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
Sequence similarities
Belongs to the strumpellin family. - Information by UniProt
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Database links
- Entrez Gene: 9897 Human
- Entrez Gene: 223593 Mouse
- Entrez Gene: 100173723 Orangutan
- Entrez Gene: 393152 Zebrafish
- Omim: 610657 Human
- SwissProt: Q12768 Human
- SwissProt: Q8C2E7 Mouse
- SwissProt: Q5R5P0 Orangutan
see all -
Alternative names
- KIAA0196 antibody
- SPG8 antibody
- STRUM_HUMAN antibody
- WASH complex subunit strumpellin antibody
Images
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Western blot - Anti-Strumpellin antibody (ab101222)All lanes : Anti-Strumpellin antibody (ab101222) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 45 kDa, 50 kDa, 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Strumpellin antibody (ab101222)ICC/IF image of ab101222 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101222 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) Hek293, HepG2, amd MCF-7 cells at 5ug/ml.
Protocols
Datasheets and documents
References (4)
ab101222 has been referenced in 4 publications.
- Farina F et al. Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis. EMBO J 38:N/A (2019). PubMed: 31015335
- Ripoll L et al. Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers. J Cell Biol 217:2709-2726 (2018). PubMed: 29875258
- Tyrrell BJ et al. Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell Melanoma Res 29:559-71 (2016). PubMed: 27390154
- Wang F et al. WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes. Sci Rep 4:5596 (2014). Mouse . PubMed: 24998208
Images
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Western blot - Anti-Strumpellin antibody (ab101222)All lanes : Anti-Strumpellin antibody (ab101222) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 45 kDa, 50 kDa, 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Strumpellin antibody (ab101222)
ICC/IF image of ab101222 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101222 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) Hek293, HepG2, amd MCF-7 cells at 5ug/ml.
