Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SF3B1 antibody [EPR11986] (ab172634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11986] to SF3B1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SF3B1 antibody [EPR11986]
    See all SF3B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11986] to SF3B1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • Positive control

    • WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11986

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
    Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
    All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : K562 lysate
    Lane 4 : Ramos lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 146 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
    Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

    Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

  • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)
    Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SF3B1 antibody [EPR11986] (ab172634)
    Anti-SF3B1 antibody [EPR11986] (ab172634)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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