Anti-SF3B1 antibody [EPR11987(B)] (ab170854)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11987(B)] to SF3B1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-SF3B1 antibody [EPR11987(B)]
See all SF3B1 primary antibodies -
Description
Rabbit monoclonal [EPR11987(B)] to SF3B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human SF3B1 (Cysteine residue). The exact sequence is proprietary.
Database link: O75533 -
Positive control
- HeLa, K562 and Ramos cell lysates; Human brain tissue and colonic carcinoma tissue; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11987(B) -
Isotype
IgG -
Research areas
Images
-
Immunofluorescent analysis of HeLa cells labeling SF3B1 with ab170854 at 1/100 dilution.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colonic carcinoma tissue labeling SF3B1 with ab170854 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling SF3B1 with ab170854 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-SF3B1 antibody [EPR11987(B)] (ab170854) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 146 kDa
-