Calmidazolium chloride, Calmodulin inhibitor (ab120658)
Key features and details
- Calmodulin inhibitor
- CAS Number: 57265-65-3
- Purity: > 98%
- Soluble in ethanol to 100 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Calmidazolium chloride, Calmodulin inhibitor -
Description
Calmodulin inhibitor -
Alternative names
- R 24571
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Biological description
Calmodulin inhibitor (IC50 = 10 nM). Elevates intracellular calcium, independent of calmodulin inhibition. Additionally inhibits adenylyl cylase activity.
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Purity
> 98% -
CAS Number
57265-65-3 -
Chemical structure
Properties
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Chemical name
3-[Bis(4-chlorophenyl)methyl]-1-[2-(2,4-dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy)ethyl]-1H-imidazolium chloride -
Molecular weight
687.70 -
Molecular formula
C31H23Cl7N2O -
PubChem identifier
644274 -
Storage instructions
Store at -20°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 100 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=CC(=CC=C1C(C2=CC=C(C=C2)Cl)N3C=C[N+](=C3)CC(C4=C(C=C(C=C4)Cl)Cl)OCC5=C(C=C(C=C5)Cl)Cl)Cl.[Cl-] -
Source
Synthetic
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Research areas
Images
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Functional Studies - Calmidazolium chloride, Calmodulin inhibitor (ab120658)ab48394 staining LC3B (LC3-II) in HeLa cells treated with calmidazolium chloride (ab120658), by ICC/IF. Increase of LC3B (LC3-II) expression correlates with increased concentration of calmidazolium chloride, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120658 (calmidazolium chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab48394 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.