Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCDH7 antibody [OTI2G6] (ab139274)

Key features and details

  • Mouse monoclonal [OTI2G6] to PCDH7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-PCDH7 antibody [OTI2G6]
    See all PCDH7 primary antibodies

  • Description

    Mouse monoclonal [OTI2G6] to PCDH7

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length Human PCDH7 (amino acids 1-1247) produced in HEK293T cells (NP_115833).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7; HepG2, HeLa, SVT2, COS7, MDCK and MCF7 cell extracts; Human endometrium tissue; COS7 cells transiently transfected with pCMV6-ENTRY PCDH7

  • General notes

    The clone number has been updated from 2G6 to OTI2G6, both clone numbers name the same clone.

    Dilute in PBS (pH7.3) before use.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 116 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206769)
  • Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 116 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206740)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling PCDH7 with ab139274 at 1/150 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (ab139274)
    Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY PCDH7 using ab139274 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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