Anti-PCDH7 antibody [OTI2G6] (ab139274)
![Anti-PCDH7 antibody [OTI2G6] (ab139274)](https://www.abcam.com/ps/products/139/ab139274/Images/ab139274-154461-anti-pcdh7-antibody-oti2g6-western-blot.jpg "Anti-PCDH7 antibody [OTI2G6] (ab139274)")
Key features and details
- Mouse monoclonal [OTI2G6] to PCDH7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-PCDH7 antibody [OTI2G6]
See all PCDH7 primary antibodies -
Description
Mouse monoclonal [OTI2G6] to PCDH7 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length Human PCDH7 (amino acids 1-1247) produced in HEK293T cells (NP_115833).
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7; HepG2, HeLa, SVT2, COS7, MDCK and MCF7 cell extracts; Human endometrium tissue; COS7 cells transiently transfected with pCMV6-ENTRY PCDH7
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General notes
The clone number has been updated from 2G6 to OTI2G6, both clone numbers name the same clone.
Dilute in PBS (pH7.3) before use.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI2G6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7
Lysates/proteins at 5 µg per lane.
Predicted band size: 116 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206769) -
![Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)](https://www.abcam.com/ps/products/139/ab139274/Images/ab139274-154460-anti-pcdh7-antibody-oti2g6-western-blot.jpg)
Western blot - Anti-PCDH7 antibody [OTI2G6] (ab139274)All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 116 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206740) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (ab139274)](https://www.abcam.com/ps/products/139/ab139274/Images/ab139274-154459-anti-pcdh7-antibody-oti2g6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCDH7 antibody [OTI2G6] (ab139274)Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling PCDH7 with ab139274 at 1/150 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (ab139274)](https://www.abcam.com/ps/products/139/ab139274/Images/ab139274-154458-anti-pcdh7-antibody-oti2g6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCDH7 antibody [OTI2G6] (ab139274)Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY PCDH7 using ab139274 at 1/100 dilution.
